Lots of splicing regulators
نویسندگان
چکیده
منابع مشابه
Identification of alternative splicing regulators by RNA interference in Drosophila.
Alternative splicing is thought to be regulated by nonspliceosomal RNA binding proteins that modulate the association of core components of the spliceosome with the pre-mRNA. Although the majority of metazoan genes encode pre-mRNAs that are alternatively spliced, remarkably few splicing regulators are currently known. Here, we used RNA interference to examine the role of >70% of the Drosophila ...
متن کاملThe splicing regulators Tra and Tra2 are unusually potent activators of pre-mRNA splicing
Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimeric complex consisting of the SR proteins Tra, Tra2 and 9G8. Using quantitative...
متن کاملRapid-response splicing reporter screens identify differential regulators of constitutive and alternative splicing.
Bioactive compounds have been invaluable for dissecting the mechanisms, regulation, and functions of cellular processes. However, very few such reagents have been described for pre-mRNA splicing. To facilitate their systematic discovery, we developed a high-throughput cell-based assay that measures pre-mRNA splicing by utilizing a quantitative reporter system with advantageous features. The rep...
متن کاملIntronic Alternative Splicing Regulators Identified by Comparative Genomics in Nematodes
Many alternative splicing events are regulated by pentameric and hexameric intronic sequences that serve as binding sites for splicing regulatory factors. We hypothesized that intronic elements that regulate alternative splicing are under selective pressure for evolutionary conservation. Using a Wobble Aware Bulk Aligner genomic alignment of Caenorhabditis elegans and Caenorhabditis briggsae, w...
متن کاملSplicing Regulators and Their Roles in Cancer Biology and Therapy
Alternative splicing allows cells to expand the encoding potential of their genomes. In this elegant mechanism, a single gene can yield protein isoforms with even antagonistic functions depending on the cellular physiological context. Alterations in splicing regulatory factors activity in cancer cells, however, can generate an abnormal protein expression pattern that promotes growth, survival, ...
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ژورنال
عنوان ژورنال: Genome Biology
سال: 2004
ISSN: 1465-6906
DOI: 10.1186/gb-spotlight-20041019-01